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TEM observation of myeloma cells treated with Abx KMS21 and KMS34 cultured with 150 μM Abx for the indicated durations (0, 12, or 48 hours). Microstructural changes in Abx-treated cells were observed by TEM. Blue arrows indicate autophagic vacuoles including autophagosomes and autolysosomes. The autophagic vacuoles per micrometer squared of 20 randomly chosen cells were counted using TEM Center software (JEOL) and are illustrated in the bar graph. Statistical significance was evaluated by Student t test. Sal, saline; Abx, ambroxol; Res, resveratrol; Baf, bafilomycin A1.

Journal: Blood Neoplasia

Article Title: Ambroxol induces myeloma cell death by inhibiting autophagy

doi: 10.1016/j.bneo.2025.100100

Figure Lengend Snippet: TEM observation of myeloma cells treated with Abx KMS21 and KMS34 cultured with 150 μM Abx for the indicated durations (0, 12, or 48 hours). Microstructural changes in Abx-treated cells were observed by TEM. Blue arrows indicate autophagic vacuoles including autophagosomes and autolysosomes. The autophagic vacuoles per micrometer squared of 20 randomly chosen cells were counted using TEM Center software (JEOL) and are illustrated in the bar graph. Statistical significance was evaluated by Student t test. Sal, saline; Abx, ambroxol; Res, resveratrol; Baf, bafilomycin A1.

Article Snippet: A JEM 1400 Plus electron microscope (JEOL, Tokyo, Japan) was used to count the number of autophagic vacuoles in 20 randomly chosen cells, and TEM Center software (JEOL) was used to measure the area of each autophagic vacuole. reveals the average autophagic vacuole area in each cell.

Techniques: Cell Culture, Software, Saline

Microstructure of the interface between HEA and Cu channel after the LMD process. a) TEM image of the HEA‐Cu interface and corresponding EDS maps: b) W, c) Ta, d) V, e) Ti. f–k) Mechanism of interface microstructure formation in the HEA‐Cu composite.

Journal: Small Methods

Article Title: Bi‐Continuous W‐Rich Refractory High Entropy Alloy‐Cu Composite: Toward Material Innovation of Nuclear Reactor Coolant System

doi: 10.1002/smtd.202500667

Figure Lengend Snippet: Microstructure of the interface between HEA and Cu channel after the LMD process. a) TEM image of the HEA‐Cu interface and corresponding EDS maps: b) W, c) Ta, d) V, e) Ti. f–k) Mechanism of interface microstructure formation in the HEA‐Cu composite.

Article Snippet: Further detailed analysis was conducted using a transmission electron microscope (TEM; JEM‐2100F, JEOL), which was also equipped with an EDS system (Ultim Max TLE, Oxford Instruments, UK) and Aztec TEM EDS software (Oxford Instruments, UK).

Techniques: